Evaluation of In Vitro Anti-Oxidant activity and Phytochemical Studies carried out for Melia Azedarach Leaf extracts
Investigation of phytochemical and biological activities was performed by Antioxidant (DPPH, H2O2, Nitric oxide) free radical scavenging methods against three gradient polarity solvents extracts of leaves of Melia azaradach Linn with different concentrations. MA species of three different assay (DPPH, H2O2, Nitric oxide) of anti-oxidant activity were showed to be IC50 values of 20.14, 35.98, 36.43 with methanolic extract of leaves were have higher concentration possess more antioxidant potential than MALEoC IC50 42.85, 54.18, 30.56 and MALEoH IC50 values of 44.68, 52.71, 48.05 when compare to reference standard ascorbic acid IC50 values of 17.87, 18.91, 25.83. They exhibited strong antioxidant DPPH, H2O2, Nitric oxide radical scavenging activities with IC50 value reference ascorbic acid, MALoME, MALoCE and MALoHE respectively. Phytoconstituents like carbohydrates, flavonoids, proteins, saponins and tannins etc. An antioxidant activity of methanol extract could be due to the presence of flavonoids and phenols. The results suggest that methanol and chloroform extracts from leaf melia azedarach tree have strong antioxidant potential.
2. Cai Y, Luo Q, Sun M, Corke H; Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004,74:2157e2184.
3. Choudhary DN, Singh JN, Verma SK, Singh BP; Antifertility effects of leaf extracts of some plants in male rats. Indian J Exp Biol,1990, 28: 714.
4. Corpinella MC , Miranda M, Almiron WR, Ferrayoli CG, Almedia FL, Palacios SM; In vitro pediculicidal and ovicidal activity of an extract and oil from fruit of melia azedarach L. J Am Acad Dermatol 2007; 56: 250-6.
5. Deepika Sharma , Yash Paul; Preliminary and Pharmacological Profile of Melia azedarach L.: An Overview. Journal of Applied Pharmaceutical Science, 2013 Vol. 3 (12), pp. 133-138.
6. Descalzo AM, Coto C; Inhibition of the pseudorabies virus (scis herpesvinyl) by an vital agent isolated from the leaves of melia azedarach Rev. Argent Microbial 1989; 21: 133-40.
7. Dickinson DA, Forman HJ; Glutathione in defense and signaling: lessons from a small thiol. Ann N Y Acad Sci. 2002;973:488e504.
8. Fadzai Boora, Elaine Chirisa, Stanley; Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents, 2014.
9. Francis M. Awahl, Andrew W, Verla; Antioxidant activity, nitric oxide scavenging activity and phenolic contents of Ocimum gratissimum leaf extract, Journal of Medicinal Plants Research, 2010, Vol. 4(24), pp. 2479-2487, ISSN 1996-0875.
10. Karlsson J; “Introduction to nutralogy Radical Formation.” In: Antioxidants and Exercise .Illinois: Human Kinetics Press, 1997, 1-143.
11. Kurata M, Suzuki M, Agar NS; Antioxidant systems and erythrocyte lifespan in mammals. Comp Biochem Physiol B. 1993;106:477e487.
12. Maxwell Afari Gyamfi, Masato Yonamine, Yoko Aniya; Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries, General Pharmacology, 32 (1999) 661–667.
13. Mohamed SA, Marzouk FA, Moharram MA, Mohamed AM, Gamal- Eldeen EA; Anticancer and antioxidant tannins from Pimenta dioica leaves, Natur Forsch, 2006;62:526e536
14. Nagulendran K, Velavan S, Mahesh R, Begum VH; In Vitro Antioxidant Activity and Total Polyphenolic Content of Cyperus Rotundus Rhizomes, 2007; 4(3): 440-449.
15. Porcher, Michel H (2012); "Melia names". Multilingual Multiscript Plant Name Database. University of Melbourne
16. Rozina Parul, Sukalayan Kumar Kundu, Pijush Saha; In Vitro Nitric Oxide Scavenging Activity Of Methanol Extracts Of Three Bangladeshi Medicinal Plants, the pharma innovate, 2013, Vol. 1 No. 12, pp.: 83, ISSN: 2277- 7695.
17. Ruch RJ, Cheng SJ, Klaunig JF; Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea, Carcinogenesis, 1989; 10: 1003–1008.