Evaluation of In Vitro Anti-Oxidant activity and Phytochemical Studies carried out for Melia Azedarach Leaf extracts

  • Surendra Babu Lagu Andhra University
  • Y. Rajendra Prasad Andhra University
  • D. Geetha Mounika Andhra University
Keywords: MALoHE, MALoCE and MALoME: Melia azadarach leaf of (hexane, chloroform and methanol) extracts, Antioxidants, DPPH: 1, 1diphenyl-2-picryl hydrazyl, Flavonoids etc

Abstract

Investigation of phytochemical and biological activities was performed by Antioxidant (DPPH, H2O2, Nitric oxide) free radical scavenging methods against three gradient polarity solvents extracts of leaves of Melia azaradach Linn with different concentrations. MA species of three different assay (DPPH, H2O2, Nitric oxide) of anti-oxidant activity were showed to be IC50 values of 20.14, 35.98, 36.43 with methanolic extract of leaves were have higher concentration possess more antioxidant potential than MALEoC IC50 42.85, 54.18, 30.56 and MALEoH IC50 values of 44.68, 52.71, 48.05 when compare to reference standard ascorbic acid IC50 values of 17.87, 18.91, 25.83. They exhibited strong antioxidant DPPH, H2O2, Nitric oxide radical scavenging activities with IC50 value reference ascorbic acid, MALoME, MALoCE and MALoHE respectively. Phytoconstituents like carbohydrates, flavonoids, proteins, saponins and tannins etc. An antioxidant activity of methanol extract could be due to the presence of flavonoids and phenols. The results suggest that methanol and chloroform extracts from leaf melia azedarach tree have strong antioxidant potential.

Author Biographies

Surendra Babu Lagu, Andhra University

Department of Pharmaceutical chemistry,
AU college of Pharmaceutical Sciences,
Andhra University, Visakhapatnam,
AP, India

Y. Rajendra Prasad, Andhra University

Department of Pharmaceutical chemistry,
AU college of Pharmaceutical Sciences,
Andhra University, Visakhapatnam,
AP, India

D. Geetha Mounika, Andhra University

Department of Pharmaceutical chemistry,
AU college of Pharmaceutical Sciences,
Andhra University, Visakhapatnam,
AP, India

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Published
2018-03-01
How to Cite
[1]
Lagu, S.B., Prasad, Y.R. and Mounika, D.G. 2018. Evaluation of In Vitro Anti-Oxidant activity and Phytochemical Studies carried out for Melia Azedarach Leaf extracts. PharmaTutor. 6, 3 (Mar. 2018), 38-44. DOI:https://doi.org/10.29161/PT.v6.i3.2018.38.
Section
Articles