Effect of Termenalia catappa Leaf Extract on Cafeteria Induced Obesity in Rats
Obesity is a leading cause of death worldwide, with increasing rates in adults and children. To investigate anti-obesity effect of Termenalia catappa leaf extract on cafeteria induced obesity in rats. For anti-obesity experiment, animals were divided into five groups of six animals and housed in cages. Normal control group continued to be fed a laboratory pellet chow ad libitum. Cafeteria diet-control group received cafeteria diet in addition to the normal pellet diet. The remaining three groups were fed with cafeteria diet and NPD along with T. catappa leaf extract (200 mg/kg, p.o.), Termenalia catappa leaf extract (400 mg/kg, p.o.) and orlistat (30 mg/kg, p.o.). Treatment was continued for 4 weeks. Animals and various adipose pads were weighed and serum total cholesterol, triglyceride, LDL, VLDL and HDL-C were measured after 4 weeks of treatment. At the end various adipose tissues, aorta were removed and processed for Histopathological study. The Termenalia catappa leaf extract -treated groups showed a significant decrease in body weight, and various adipose pad weight and serum TC, TG, LDL, VLDL and increase in HDL levels after 4 weeks treatment and decrease in the adipose tissue size and adipocyte number. At present study, Termenalia catappa leaf extract can inhibit the development of obesity and hyperlipidemia on cafeteria induced obesity in rat. It is because of various phytoconstituents of leaf extract. But further studies are still waiting for establishing mechanism and isolation of phytoconstituents. By observing above results Termenalia catappa leaf extract can act as adjuvant in obesity treatment.
2. Aniket Karmase, Rahul Birari, Kamlesh K. Bhutani. Evaluation of anti-obesity effect of Aegle marmelos leaves. Phytomedicine . 2013; 20:805– 812.
3. Birari, R.B., Bhutani, K.K., 2007. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discovery Today 12, 879–889
4. Birari, R.B., Gupta, S., Mohan, C.G., Bhutani, K.K., 2011. Antiobesity and lipid lowering effects of Glycyrrhiza chalcones: experimental and computational studies. Phytomedicine 18, 795–80
5. C.K.Kokate. Practical Pharmacognosy.4th Ed. New Gyan Offset Printers, Delhi.1994, Pp 128-135
6. D.B.F. Carla, F.B. Fernanda, S.A.F. Glaura., Diet-induced obesity in rats leads to a decrease in sperm motility. Rep. Biol. Endocrinol. 2011; 9: 32–42.
7. D.T. Villareal, S. Chode, N. Parimi, D.R. Sinacore, T. Hilton, V.R. Armamento, N. Napoli, Q. Clifford, S. Krupa. Weight loss, exercise, or both and physical function in obese older adults. New Eng. J. Med. (2011) 364; 1218–1229
8. Gamal A. Mohamed, Sabrin R.M. Ibrahim, Ehab S. Elkhayat ,Riham Salah El Dine. Review on Natural anti-obesity agents. Bulletin of Faculty of Pharmacy, Cairo University.2014; 52: 269–284.
9. Harris RB. The impact of high or low fat cafeteria foods on nutrient intake and growth of rats consuming a diet containing 30 % energy as fat. Int J Obes Relat Metab Disord. 1993; 17:307-15.
10. I.J.N. Padmavathi, Y.D. Kishore, L. Venu, M. Ganeshan, N. Harishankar, N.V. Giridharan, M. Raghunath, Prenatal and perinatal zinc restriction.: effects on body composition, glucose tolerance and insulin response in rat offspring, Exp. Physiol(2009). 94; 761–769.
11. Khandelwal KR. Practical Pharmacognosy –techniques and experiments. 16th Ed. Nirali prakashan, Pune. 2006,Pp 149-156.
12. Kuller LH. Dietary fat and chronic diseases: Epidemiologic overview. J Am Diet Assoc 1997;97:S9-15.
13. Kusunoki M, Hara T, Tsutsumi K, Nakamura T, Miyata Y, Sakakibara F, et al. The lipoprotein lipase activator, NO-1886, suppresses fat accumulation and insulin resistance in rats fed a high fat diet. Diabetologia 2000; 43:875-80.
14. Pankaj Oudhia, Robert E. Paull. West Indian Almond Termenalia catappa L. Combretaceae of Fruit and Nuts - 2008, J. Janick and R. E. Paull -editors, CABI, Wallingford, United Kingdom.
15. Saurabh arjariya, nitin nema, swati tiwari. Investigate the toxicological effect on aqueous extract of terminalia catappa linn. in rat. International journal of research and development in pharmacy and life sciences. 2013; 5: 596-601.
16. Shiv Kumar, K.R. Alagawadi1, M. Raghavendra Rao. Effect of Argyreia speciosa root extract on cafeteria diet-induced obesity in rats. Indian Journal of Pharmacology .2011; 43: 163.
17. Z. Min, Z. Baocai, C. Mengjie. Differential responses of hepatic endoplasmic reticulum stress and inflammation in diet-induced obese rats with high-fat diet rich in lard oil or soybean oil.PLoS One (2013) 28; e78620–e78632.