Determination of Antibacterial, Antifungal and Cytotoxic activities of n-Hexane, Chloroform and Ethyl Acetate extracts of Momordica charantia leaves

  • Israt Jahan Bulbul Southeast University Banani
Keywords: M. charantia, antibacterial, MIC, cytotoxic

Abstract

A study was conducted to determine the antibacterial and antifungal activities with minimum inhibitory concentration and cytotoxic activity of Momordica charantia (Family: Cucurbitaceae) leaves. In our present study, the antimicroial activity of n-hexane, chloroform and ethyl acetate fractions of the plant were investigated against a number of pathogenic Gram-positive (Bacillus megaterium, Bacillus subtilis, Staphylococcus aureus and Sarcina lutea), Gram- negative (Salmonella paratyphi, Salmonella typhi, Vibrio parahaemolyticus, Vibrio mimicus, Escherichia coli, Shigella dysenteriae, Shigella boydii and Pseudomonas aeruginosa) bacteria and three funguses (Candida albicans, Aspergillus niger and Saccharomyces cerevesiae). Here the zones of inhibitions for the test samples (500 µg /disc) were compared with that of reference standard (30 µg /disc) in determining antimicrobial activity. All the extracts showed significant antibacterial and antifungal activities against all the pathogenic bacteria except A. niger. The highest sensitivity for n-hexane, chloroform and ethyl acetate fractions was against gram positive bacteria B. cereus. Almost all the gram positive, gram negative bacteria and fungus were inhibited by ethyl acetate extract and showed better activity compared to n-hexane and chloroform extracts. All the three fractions were tested as antifungal against C. albicans and S. cerevesiae. They showed moderate activity against C. albicans whereas a very good activity against S. cerevesiae. But A. niger was not sensitive to the experimental extracts. Minimum inhibitory concentration (MIC) that is the lowest concentration at which the test sample shows its highest activity against microorganisms was tested by serial dilution method. The MIC for n-Hexane and chloroform extracts was against B. cereus (64 µg /ml).  The ethyl acetate extract exhibited antibacterial activity with MIC of 64 µg /ml against S. aureus, S. luteae, S. boydii, S. dysentereae and V. mimicus. The Brine shrimp lethality bioassay method was used to determine the cytotoxic activity and vincristine sulphate was used as positive control. The LC50 values of standard vincristine sulphate, n-hexane, chloroform and ethyl acetate extract were 10.18 µg /ml, 24.71 µg /ml, 19.02 µg /ml and 30.38 µg/ml respectively which indicate the presence of bioactive compounds present in the plant extracts are promisingly cytotoxic.

Author Biography

Israt Jahan Bulbul, Southeast University Banani

Department of Pharmacy,
Southeast University Banani,
Dhaka, Bangladesh

References

1. Duke J. CRC Handbook of Medicinal Herbs. Boca Raton, FL; CRC Press Inc; 1989; 315-316.
2. Horax R, Hettiarachchy N, Chen P; Extraction, quantification, and antioxidant activities of phenolics from pericarp and seeds of bitter melons (Momordica charantia) harvested at three maturity stages (immature, mature, and ripe); J Agric Food Chem; 2010; 58(7); 4428-4433.
3. Braca A, Siciliano T, D'Arrigo M, Germanò MP; Chemical composition and antimicrobial activity of Momordica charantia seed essential oil; Fitoterapia; 2008; 79(2); 123-125.
4. Li QY, Liang H, Wang B, Zhao YY; Chemical constituents of Momordica charantia L; Yao Xue Xue Bao; 2009; 44(9); 1014-1018
5. José Galberto M. Costa, Eidla M. M. Nascimento, Adriana R. Campos and Fabiola F. G. Rodrigues; Antibacterial activity of Momordica charantia (Curcubitaceae) extracts and fractions; Journal of Basic and Clinical Pharmacy, 2011; 2(1); 45-53.
6. Asli SEMIZ, Alaattin SEN; Antioxidant and chemoprotective properties of Momordica charantia L. bitter melon fruit extract; African Journal of Biotechnology; 2007; 6(3)273-277.
7. Yadav Umesh CS, Moorthy K, Baquer Najma Z; Combined treatment of sodium orthovanadate and Momordica charantia fruit extract prevents alterations in lipid profile and lipogenic enzymes in alloxan diabetic rats; Molecular and Cellular Biochemistry; 2005; 268(1); 111-120.
8. Vikrant V, Grover JK, Tandon N, Rathi SS, Gupta N; Treatment with extracts of Momordica charantia and Eugenia jambolana prevents hyperglycemia and hyperinsulinemia in fructose fed rats; J Ethnopharmacol; 2001; 76(2); 139-143.
9. Khan SA; Bitter gourd (Momordica charantia): A potential mechanism in anti-carcinogenesis of colon; World Journal of Gastroenterology; 2007; 13(11); 1761-1762.
10. Rebultan SP; Bitter melon therapy: an experimental treatment of HIV infection; AIDS Asia, 1995; 2(4), 6-7.
11. Nerurkar PV, Pearson L, Efird JT, Adeli K, Theriault AG, Nerurkar VR; Microsomal triglyceride transfer protein gene expression and ApoB secretion are inhibited by bitter melon in HepG2 cells; Journal of Nutrition; 2005; 135(4); 702-706.
12. Carson CF, Hammer KA, Riley TV; Broth micro-dilution method for determination of susceptibility of Escherichia coli and Staphylococcus aureus to the essential oil of Malaleuca alterifolia (Tea tree oil); Microbios; 1995; 82(332); 181-185.
13. Iwaki K, Koya-Miyata S, Kohno K, Ushio S, Fukuda S; Antimicrobial activity of Polygonum tinctorium Lour; Extract against oral pathogenic bacteria; Journal of Natural Medicines; 2006; 60(2); 121-125.
14. Khan A, Rahman M, Islam S; Antibacterial, antifungal and cytotoxic activities of Tuberous Roots of Amorphophallus campanulatus; Turk. J. Biol.; 2007; 31; 167-172.
15. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL; Brine shrimp; a convenient general bioassay for the active plant constituents; Planta Medicine; 1982; 45; 31-34.
16. Zhao GX, Y.-H. Hui, Rupprecht JK, McLaughlin JL, Wood KV; Additional bioactive compounds and trilobacin, a novel highly cytotoxic acetogenin, from the bark of Asimina triloba; Journal of Natural Products; 1992; 55(3); 347-356.
17. T. A. Geissman, Flavonoid compounds, tannins, lignins and related compounds, p. 265. In M. Florkin and E. H. Stotz (ed.), Pyrrole pigments, isoprenoid compounds and phenolic plant constituents, vol. 9. Elsevier, New York, N.Y, 1963.
18. R. E. Schultes, The kingdom of plants, p. 208. In W. A. R. Thomson (ed.), Medicines from the Earth. McGraw-Hill Book Co., New York, N.Y., 1978.
19. H Tsuchiya, M. Sato, T. Miyazaki, S. Fujiwara, S. Tanigaki, M. Ohyama, T. Tanaka, and M. Iinuma; Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant S. aureus; J. Ethnopharmacol; 1996; 50(1); 27-34.
20. C.Ya, S. H. Gaffney, T. H. Lilley, and E. Haslam. Carbohydrate polyphenol complexation, p. 553. In R. W. Hemingway and J. J. Karchesy(ed.), Chemistry and significance of condensed tannins. Plenum Press, New York, N.Y., 1988.
Published
2016-03-01
How to Cite
[1]
Bulbul, I. 2016. Determination of Antibacterial, Antifungal and Cytotoxic activities of n-Hexane, Chloroform and Ethyl Acetate extracts of Momordica charantia leaves. PharmaTutor. 4, 3 (Mar. 2016), 28-33.
Section
Articles