Formulation and evaluation of Gastroretentive hydrochlorothiazide Floating Microspheres: Statistical Analysis

  • Y. Veda Pravallika Bapatla college of pharmacy
  • K. Rajyalakshmi Bapatla college of pharmacy
Keywords: Hydrochlorothiazide, Gastro retentive drug delivery systems, floating microspheres, orifice-ionic gelation, sodium alginate

Abstract

The main objective of this work is to formulate gastroretentive floating microspheres of hydrochlorothiazide and to study the effect of formulation variables like drug to polymer ratio and concentration of polymer dispersion. The hydrochlorothiazide floating microspheres were formulated by using orifice-ionic gelation technique. These microspheres were formulated by using sodium alginate as sustained release polymer, sodium bicarbonate as gas generating agent and calcium chloride as a cross linking agent. The rheological properties of the polymer dispersion were studied. The microspheres were formulated by different drug to polymer ratio’s at various concentrations of sodium alginate dispersion. Thus formulated microspheres were evaluated for floating behaviour, drug entrapment efficiency, drug content, micromeritic properties, particle size, swelling index, invitro drug release studies and release kinetics. The better formulation was subjected for stability studies and SEM analysis. The effect of formulation variables on the entrapment efficiency and release rate constant was studied by statistical analysis. The hydrochlorothiazide microspheres formulated with 1:3 drug to polymer ratio and 3.5% sodium alginate dispersion concentration was selected as optimized formulation based on the results.

Author Biographies

Y. Veda Pravallika, Bapatla college of pharmacy

Bapatla college of pharmacy, Bapatla,
Andhra Pradesh, India

K. Rajyalakshmi, Bapatla college of pharmacy

Bapatla college of pharmacy, Bapatla,
Andhra Pradesh, India

References

1. Rouge N, Buri P, Doelkar E. Drug absorption sites in the gastrointestinal tract and dosage for site specific delivery. Int J Pharm. 1996; 136: 117-139.
2. Umamaheshwari RB, Jain S, Bhadra D. Floating microspheres bearing Acetohyxamic acid for the treatment of Helicobacter pylori. J Pharm Pharmacol. 2003;55: 1607-1613.
3. Jain SK, Awasthi AW, Jain NK, et al. Calcium silicate based microspheres of Repaglinide for gastroretentive floating drug delivery: Preparation and in vitro characterization. J Control Release 2005;107: 300-309.
4. Sanjay J. Kshirsagar, Sharadachandra B. Wadekar, Magesh R. Bhalekar, Pallavi B. Ughade, Aswini R. Madgulkar. Gastroretentive drug delivery system of hydrochlorothiazide: Formulation, optimization and invivo evaluation. Asian journal of pharmaceutical sciences 2011;6(3-4):166-175.
5. A. Prasanth kumar. Formulation development of hydrochlorothiazide floating microspheres. International journal of pharmaceutical sciences review and research 2014;25(1):274-280.
6. Hardman JG, Limbird LE, Gilman AG. The Pharmacological basis of therapeutics. New York, McGraw Hill; 2001:pp 774.
7. James WA. Expandable gastric retention device. 2004, US Patent US2004/0219186A1.
8. Choi BY, Park J, Hwang SJ and Park JB. Preparation of alginate beads for floating drug delivery system: effects of CO2 gas-forming agents. International Journal of Pharmaceutics 2002; 239:81–91.
9. N.K.Jain. Advances in controlled and novel drug delivery. First Edition CBS publishers& distributors, New Delhi, 2001;pp 89-112.
10. Elkheshen SA, Eldeen B, Alsuwayeh S, AlkhaIed A. In vitro and in vivo Evaluation of Floating Controlled Release Dosage Forms, Pharm. Ind. 2004;66: 1364-1372.
11. Klausner EA, LavyV, Friedman M, Hoffman A; Expandable gastroretentive dosage forms, J of Control; 2003; 90(2); 143–162.
12. Aphale Sanjivani, Shinde Swapnila, Dhat Shalaka, Bagul Uddhav and Saluja Jagdish. Development and evaluation of hollow microspheres of clarithromycin as a gastroretentive drug delivery system using eudragit polymers. International Journal of Pharma and Bio Sciences 2011;2(3):344-358.
13. Ghulam Murtaza. A comparative study of various micro encapsulation techniques effect of polymer viscosity on microcapsule characteristics. Pak. J. Pharm. Sci. 2009;22(3):291-300.
14. Dandagi P.M et al. Microencapsulation of Verapamil Hydrochloride by Ionotropic gelation technique, Ind. J. Pharm. Sci. 2004;66 (5 ); 631-635.
15. Alf Lamprechet, Ulrich Schafer, and Claus- Michael Lehr. Structural analysis of micropartcles by Confocal laser Scanning Microscopy, AAPS Pharm SciTech. 2000;1(3):17-27.
16. Mohpatra A, Parikh RK, Gohel MC. Formulation, development and evaluation of patient friendly dosage form of Metformin, part-III: soluble effervescent tablets. Asian J Pharm. 2008;177-81.
17. Bhaskar Mazumder, Mrinal Kanti Sarkar, Sanay Dey, Nibedita Roy. Effect of formulation and process variables on the charecteristics of microspheres of anti-viral drug (Stavudine) prepared by oil-in-oil solvent evaporation technique. International journal of pharmacy and pharmaceutical sciences 2010;2(2):52-57.
Published
2016-01-01
How to Cite
[1]
Pravallika, Y. and Rajyalakshmi, K. 2016. Formulation and evaluation of Gastroretentive hydrochlorothiazide Floating Microspheres: Statistical Analysis. PharmaTutor. 4, 1 (Jan. 2016), 28-36.
Section
Articles